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crown_gall
has definitions from the field of medicine
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[ noun ] (medicine) a bacterial disease of plants (especially pome and stone fruits and grapes and roses) which forms excrescences on the stem near the ground
Used in print (A. N. Nagaraj and L. M. Black, "Localization of...)In attempting to improve specificity of staining , the fluorescein labeled antisera used in both direct and indirect methods were treated in one of several ways : ( 1 ) They were passed_through Dowex-2-chloride twice and treated with acetone insoluble powders ( Coons , 1958 ) prepared from mouse liver or from healthy sweet_clover stems or crown_gall tissue produced by Agrobacterium_tumefaciens ( E._F._Smith + Townsend ) Conn , on sweet_clover stems . Sections of crown_gall tissue similarly stained with either * * f and * * f or NS and * * f also showed only the light_green nonspecific fluorescence . Two absorptions of * * f with ethyl_acetate or two absorptions of * * f ( which had been passed_through Dowex-2-chloride ) , NS and * * f with crown_gall tissue powder , or mouse liver powder did not further improve the specificity of staining . After these treatments the conjugate did not stain healthy or crown_gall sweet_clover tissues or stained them a very faint green which was easily distinguishable from the bright yellow-green specific staining . The specificity of staining in WTV tumors with * * f and * * f but not with NS and * * f or with antiserum to potato_yellow-dwarf_virus and * * f , and the absence of such staining in crown_gall tumor tissue from sweet-clover , indicate that an antigen of WTV was being stained . Related terms |
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