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[ noun ] centrifugation at very high speeds
Used in print (M. Yokoyama et al., "Chemical and serological...)These include low-temperature ethanol ( Cohn ) fractionation , electrophoresis , ultracentrifugation and column_chromatography on ion_exchange celluloses . Fractions from the column which were to be subjected to analytical ultracentrifugation were concentrated by negative pressure dialysis and dialyzed for 16 hr in the cold against at_least 500 volumes of phosphate buffered saline , pH 7.2 , ionic strength 0.154 . For preparative ultracentrifugation , fractions from the column were concentrated by negative pressure dialysis to volumes of 1 ml or less , transferred to cellulose tubes and diluted to 12 ml with isotonic saline . Ultracentrifugation was then carried_out in a Spinco model L ultracentrifuge at 40000 rpm for 125 to 150 min , refrigeration being used throughout the run . Related terms |
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